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Characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer Mazama gouazoubira Genet. Mol. Biol.
Caparroz,Renato; Mantellatto,Aline M.B.; Bertioli,David J.; Figueiredo,Marina G.; Duarte,José Maurício B..
The complete mitochondrial genome of the brown brocket deer Mazama gouazoubira and a set of polymorphic microsatellite markers were identified by 454-pyrosequencing. De novo genome assembly recovered 98% of the mitochondrial genome with a mean coverage of 9-fold. The mitogenome consisted of 16,356 base pairs that included 13 protein-coding genes, two ribosomal subunit genes, 22 transfer RNAs and the control region, as found in other deer. The genetic divergence between the mitogenome described here and a previously published report was ∼0.5%, with the control region and ND5 gene showing the highest intraspecific variation. Seven polymorphic loci were characterized using 15 unrelated individuals; there was moderate genetic variation across most loci (mean...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Microsatellites; Mitogenome; Population genetics; 454-pyrosequencing.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300338
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The comparative mitogenomics and phylogenetics of the two grouse-grasshoppers (Insecta, Orthoptera, Tetrigoidea) Biol. Res.
Sun,Yufang; Liu,Dianfeng; Xiao,Bo; Jiang,Guofang.
Abstract Objective This study aimed to reveal the mitochondrial genomes (mtgenomes) of Tetrix japonica and Alulatettix yunnanensis, and the phylogenetics of Orthoptera species. Methods The mtgenomes of A. yunnanensis and T. japonica were firstly sequenced and assembled through partial sequences amplification, and then the genome organization and gene arrangement were analyzed. Based on nucleotide/amino acid sequences of 13 protein-coding genes and whole mtgenomes, phylogenetic trees were established on 37 Orthoptera species and 5 outgroups, respectively. Results Except for a regulation region (A+T rich region), a total of 37 genes were found in mtgenomes of T. japonicaand A. yunnanensis, including 13 protein-coding genes, 2 ribosomal RNA genes, and...
Tipo: Journal article Palavras-chave: Mitogenome; Orthoptera Tetrigoidea; Phylogenetic.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100219
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The complete mitochondrial genome of Corydoras nattereri (Callichthyidae: Corydoradinae) Neotropical Ichthyology
Moreira,Daniel A.; Buckup,Paulo A.; Britto,Marcelo R.; Magalhães,Maithê G. P.; Andrade,Paula C. C. de; Furtado,Carolina; Parente,Thiago E..
ABSTRACT The complete mitogenome of Corydoras nattereri , a species of mailed catfishes from southeastern Brazil, was reconstructed using next-generation sequencing techniques. The mitogenome was assembled using mitochondrial transcripts from the liver transcriptomes of three individuals, and produced a circular DNA sequence of 16,557 nucleotides encoding 22 tRNA genes, two rRNA genes, 13 protein-coding genes and two noncoding control regions (D-loop, OrigL). Phylogeographic analysis of closely related sequences of Cytochrome Oxydase C subunit I (COI) demonstrates high diversity among morphologically similar populations of C. nattereri . Corydoras nattereri is nested within a complex of populations currently assigned to C. paleatus and C. ehrhardti ....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Barcode; DNA; Mitogenome; Molecular diversity; MtRNA..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000100218
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The complete mitochondrial genome of the threatened Neotropical catfish Lophiosilurus alexandri (Silurifomes: Pseudopimelodidae) and phylogenomic analysis indicate monophyly of Pimelodoidea Genet. Mol. Biol.
Carvalho,Daniel Cardoso; Perini,Violeta da Rocha; Bastos,Alex Schomaker; Costa,Igor Rodrigues da; Luz,Ronald Kennedy; Furtado,Carolina; Prosdocimi,Francisco.
Abstract Lophiosilurus alexandri is an endemic catfish from the São Francisco River Basin (Brazil) popularly known as pacamã, which has economic potential for aquaculture farming. The mitochondrial genome was sequenced for the threatened Neotropical catfish L. alexandri. Assembly into scaffolds using MIRA and MITObim software produced the whole, circularized mitochondrial genome, which comprises 16,445 bp and presents the typical gene arrangement of Teleostei mitochondria. A phylogenomic analysis was performed after the concatenation of all proteins obtained from whole mitogenomes of 20 Siluriformes and two outgroups. The results confirmed the monophyly of nine families of catfishes and also clustered L. alexandri as a sister group to the family...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Mitogenome; Fish; Next-generation-sequencing; Illumina; Pseudopimelodidae.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400674
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The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of Drosophila Genet. Mol. Biol.
Oliveira,Daniel S.; Gomes,Tiago M.F.F.; Loreto,Elgion L.S..
Abstract The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae) was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced) and a partial A+T-rich region. All protein-coding genes start with ATN codons. Eleven protein-coding genes presented TAA stop codons, whereas ND6 and COII that presented TA, and T nucleotides, respectively. The gene pattern revealed extensive rearrangements compared to the typical pattern generally observed in insects. These rearrangements involve two...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Mitogenome; Cynipoidea; Leptopilina; Parasitic wasp.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400611
Registros recuperados: 5
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